Cellular and ultrastructural alterations of Arabidopsis thaliana roots in response to exogenous trans-aconitic acid

Abstract In this work, the responses of Arabidopsis thaliana (L.) Heynh to trans -aconitic acid (TAA) were investigated. A. was grown in presence TAA a concentration range 400–1200 µM for 7 or 15 days. Changes morphoanatomy, cellular ultrastructure, and micromorphology roots evaluated by light transmission electron (TEM) microscopy. At concentrations below 1000 µM, reduced length primary roots, but induced an early appearance lateral root hairs. 1200 suppressed growth seedlings. The images longitudinal sections tips seedlings treated with IC 50 (684 µM) revealed elongation zone increased differentiation zone. TEM showed increase number volume vacuoles, vesicles containing electron-dense material derived from plasmalemma, granules attached cell wall. Trans suggesting interference auxin action. ultrastructure may represent vacuolar extracellular accumulation TAA, remove excess cytosol mitochondria. An inhibition aconitase chelation intracellular cations have contributed cytotoxicity at concentration.